Klein, P. D. et al.: A study of the onset of unsaturated fatty acid deficiency in subcellular particles of rat livers

Peter D. Klein, Ralph M. Johnson: A study of the onset of unsaturated fatty acid deficiency in subcellular particles of rat livers.
In: Archives of Biochemistry and Biophysics, Volume 48, Issue 2, February 1954, Pages 380–385.

Artikel, kostenpflichtig: http://www.sciencedirect.com/science/article/pii/0003986154903537

Zitat :

Abstract

1. Rats placed on a fat-free diet lose di-, tetra-, and pentaenoic acid from cytoplasmic particles at a high initial rate which declines by 6 weeks. Thereafter, the losses proceed very slowly, in some cases without change for 12–24 weeks.

2. The largest losses are found in the microsomal fraction and the least in the mitochondrial, while those of the poorly sedimentable fraction are intermediate.

3. The trienoic acid accumulates in all three fractions, reaching levels of four to six times those found in the fraction under normal conditions.

4. Since these alterations occur approximately 6 weeks before the onset of external symptoms of fat deficiency, it is suggested that the primary lesion in fat deficiency may take place earlier than heretofore suspected and may bear only a secondary relationship to the external symptoms.